1. komponenta

Lecture type	Total
Laboratory exercises	60

* Load is given in academic hour (1 academic hour = 45 minutes)

COURSE OBJECTIVE: Through experimental exercises the student gets acquainted with the work and methods of work on modern
instrumentation (in all laboratories included in the Applied Chemistry study).

COURSE EXECUTION PROGRAM (elaborate them as precisely as possible according to the teaching weeks):
Exercises are done in a block of 7 weeks, two days a week (average 5.36 hours per day) within
a total estimated hourly rate of 75 hours. Students in groups visit all Departments included in the course and
do the exercises using existing instruments according to the following schedule:
ZOK (2 weeks = 21 hours): Monitoring reactions using GC / MS,
UV / VIS, Fluorescence and HPLC;
ZAK (2 weeks = 21 hours): Chromatography (HPLC / MS, Ion chromatography),
Atomic absorption spectrometry;
ZEK (2 weeks = 21 hours): Voltammetry, EIS, Quartz-crystalline nanoga;
ZOAK (1 week = 12 hours): ICP (Inductively coupled plasma),
Integrated analytical systems.
At the end of each completed exercise, ie problem solving using a certain technique, the code is collocated
teacher / assistant and writes a paper.

DEVELOPMENT OF GENERAL AND SPECIFIC COMPETENCIES OF STUDENTS:
Through experimental work, students get acquainted with the principle of working on modern instruments and acquire practical skills,
advantages and limitations of certain techniques and methods in different areas of their application.
STUDENTS 'TEACHING OBLIGATIONS AND THEIR PERFORMANCE:
Students are required to work on the exercises and at the end of the exercise to colloquize and submit a paper.

CONDITIONS FOR OBTAINING A SIGNATURE:
Work on exercises.

TEACHING METHODS:
Laboratory exercises on instruments.

METHOD OF EXAMINATION OF KNOWLEDGE AND EXAMINATION:
Colloquia and papers.

METHOD OF MONITORING THE QUALITY AND PERFORMANCE OF COURSES:
Student survey

METHODOLOGICAL REQUIREMENTS: Enrolled Final Thesis.

Course learning outcomes:
1. be able to explain the basic principles of operation of individual instrumental methods of analysis;
2. be able to choose a suitable analytical method for a particular problem;
3. be able to understand the advantages, disadvantages and limitations of each method;
4. gain the necessary experience and knowledge to use the instrument;
5. be able to analyze the obtained data on certain instruments;

program-level learning outcomes:
1. apply a certain instrumental technique in the analysis of a given substrate;
2. use the acquired knowledge in the production process and quality control;
3. apply the acquired knowledge in research projects;
4. apply computer skills and data processing related to chemical data and information;

Teaching units with associated learning outcomes and evaluation criteria

Teaching unit
ZAK
1. Analytical approach to chemical analysis from sample preparation to interpretation of results - application to environmental samples

Learning outcomes
- introduction and acquisition of knowledge about the principles of work on instruments for analysis of samples from the environment;
- determination of inorganic and organic analyte in water samples (drinking and waste) by chromatographic methods (HPLC-DAD and IC) and atomic absorption spectrometry (AAS).

Evaluation criteria
- distinguish the approach to the analysis of samples by spectrometric and chromatographic methods;
- distinguish the principle of individual methods;
- ability to independently select and apply the appropriate calibration method and create a calibration direction;
- show independence in work;
- numerically express and process the results based on the obtained measurements;
- keep a laboratory diary.

Teaching unit
ZOK
2. Introduction to the basic principles of work on GC / MS, UV / Vis spectrophotometer, fluorimeter, HPLC / MS, and microwave reactor, especially in the analysis and synthesis of organic samples

Learning outcomes
- get acquainted with the basic principles of operation of the connected system gas chromatograph / mass spectrometer (GC / MS), UV / Vis spectrophotometer and fluorimeter, HPLC / MS system;
- get acquainted with the application of organic synthesis assisted by microwaves;
- gain knowledge for the use of a particular instrument;
- be able to combine all techniques in the analysis of the reaction mixture;

Evaluation criteria
- conclude on the advantages, disadvantages and limitations of working on a particular instrument;
- discuss the obtained data with a certain technique;
- analyze the spectra of organic compounds used in various instrumental techniques;
- judge which instrumental method is more favorable depending on the analyzed structures;

Teaching unit
ZEK
3. Research of chemical systems by electrochemical techniques (cyclic voltammetry, electrochemical quartz crystal nano-scale, electrochemical impedance spectroscopy, hydrodynamic voltammetry).

Learning outcomes
- get acquainted with the basic principles of electrochemical techniques
- interpret the obtained results
- to acquire knowledge and skills necessary for the application of electrochemical techniques in chemical analysis but also in synthesis.

Evaluation criteria
- show the ability to work independently and interpret electrochemical results;
- to connect the results obtained by electrochemical techniques with the mechanism of appropriate physico-chemical processes and reactions;
- distinguish the principles of operation of electrochemical techniques

Teaching unit
ZOAK
4. Application of ICP-MS techniques in element analysis.

Learning outcomes
- get acquainted with the basic principles of operation and possibilities of application of ICP-MS techniques in the analysis of elements
- get acquainted with the principles of qualitative and quantitative analysis of elements and isotopic composition of elements
- master the basic principles of instrument calibration
- master the basic principles of sample preparation for analysis
- identify possible interferences in the analysis of elements.

Evaluation criteria
- distinguish the principles of operation of atomic absorption, emission and mass spectrometers
- explain the principles of operation of ICP-MS techniques
- distinguish between low and high resolution spectrometers
- explain the principles of removing interference using a dynamic reaction chamber
- demonstrate the ability to prepare samples for analysis and to calculate and interpret the results obtained.

1. 1. D. A. Skoog, J. J. Leary, Principles of Instrumental Analysis, Saunders College Publishing, Fort Worth, 1992.
   2. L. D. Field, S. Sternhell, J. R. Kalman, Organic Structures from Spectra, John Wiley & Sons, Inc., New York,
   USA, 2003.
   3. E. Pretsch, P. Buehlmann, C. Affolter, Structure Determination of Organic Compounds, Springer, Berlin, 2003.
   4. F. Scholz, Electroanalytical Methods: Guide to Experiments and Applications, Springer, Berlin, 2002.
   5. A.J. Bard i L.R. Faulkner, Electrochemical methods: Fundamentals and applications, John Wiley and Sons, New
   York, 1980,

Enrollment :
Passed : Analytical chemistry I
Passed : Basics of electrical engineering
Passed : Basics of mechanical engineering
Passed : Calculus II
Passed : Computer programming and application
Passed : General chemistry
Passed : Inorganic chemistry
Passed : Physics II

Mandatory course - Regular studij - Applied Chemistry