COURSE OBJECTIVE:
To acquaint students with the physical basis of molecular spectroscopies and with the application of each method in chemistry individually, especially in the interpretation of spectra in determining the structures of organic compounds.
COURSE EXECUTION PROGRAM (elaborate them as precisely as possible according to the teaching weeks):
1. IR spectroscopy - application in determining the structures of organic compounds
2. UV / Vis spectroscopy and fluorescence: instrumentation, presentation of spectra, solvents, chromophores, conjugation effect
3. NMR spectroscopy: basic approaches, nuclear magnetic moment. 1H NMR spectra: chemical shift and shielding, integrals, chemical environment and chemical shift, magnetic anisotropy, coupling constant
4. NMR spectroscopy. 13C NMR spectra: chemical shifts of carbon-13, integration in 13C NMR spectrum, NOE effect, heteronuclear coupling of carbon with deuterium, fluorine-19 and phosphorus-31
5. NMR spectroscopy. Spin-spin coupling: coupling mechanism, first and second order spectrum coupling constants, long range couplings
6. NMR spectroscopy. Additional chapters in one-dimensional NMR: proton exchange in water and D2O, tautomerism, protons on the nitrogen atom, influence of solvent on chemical shift; Advanced NMR techniques: DEPT experiment, two-dimensional spectroscopic methods, COSY, HETCOR
7. Mass spectrometry: mass spectrometer, GC / MS, mass spectrum, determination of molecular weight and formula, influence of isotopes
8. Mass spectrometry: fragmentation
DEVELOPMENT OF GENERAL AND SPECIFIC COMPETENCIES OF STUDENTS:
The student is trained to, by combining the spectroscopic methods he is introduced to in the course, be able to analyze the obtained results and apply them in determining the structure of organic compounds.
STUDENTS 'TEACHING OBLIGATIONS AND THEIR PERFORMANCE:
Students are required to attend classes and solve tasks independently.
CONDITIONS FOR OBTAINING A SIGNATURE:
Regular class attendance
TEACHING METHODS:
Lectures and seminar assignments. Students must learn to solve tasks independently
METHOD OF EXAMINATION OF KNOWLEDGE AND EXAMINATION:
The exam can be passed through colloquia that are held after each major methodological unit. Students who do not pass the colloquia (4 in total) take a written exam.
METHOD OF MONITORING THE QUALITY AND PERFORMANCE OF COURSES:
Student survey
COURSE LEARNING OUTCOMES:
1. be able to explain the physical basis of individual molecular spectroscopies;
2. be able to choose the appropriate spectroscopic method;
3. extract relevant data from the given spectra;
3. define structural units based on the selected spectroscopic method;
4. be able to correlate the obtained data;
5. combine individual spectroscopic methods;
6. develop a logical approach to solving with proposing an acceptable structure for given spectroscopic data;
LEARNING OUTCOMES AT PROGRAM LEVEL:
1. apply spectroscopic methods in the analysis of a given substrate;
2. use spectroscopic methods in monitoring the reaction process;
3. apply the acquired knowledge in research projects;
4. ability to select appropriate spectroscopic methods in monitoring the use of various materials and in critical analysis of results;
TEACHING UNITS WITH ASSOCIATED LEARNING OUTCOMES AND EVALUATION CRITERIA
1. Physical basis of molecular spectroscopy
Learning outcomes
-determine the type of interaction of electromagnetic radiation and matter for each individual spectroscopic method
- explain how to detect the signal
Evaluation criteria
- determine the appropriate spectroscopic method
- determine the number of expected spectroscopic bands, their shape, half-width and intensity
2. Various spectroscopic methods (IR, UV / VIS, MS, NMR)
Learning outcomes
- define the waveband;
- identify functional groups and chromophores from IR or UV / VIS spectra;
- determine the molecular ion and find the characteristic fragments in the MS spectrum;
- assign signals in 1H and 13C spectra to appropriate structural units;
- be able to propose a compound structure based on spectral data;
Evaluation criteria
- recognize and interpret the spectra of simple molecules;
- determine the structure of the compound based on the given spectra;
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Introduction to Spectroscop, Fifth Edition, Brooks-Cole Thomson Learning, Australia, 2015. (4th 2008, 3rd 2001), D. L. Pavia et al, 2015.
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Structure Determination of Organic Compounds, Tables of Spectral data, Third Edition, Springer-Verlag Berlin Heidelberg, E. Pretsch, P. Buehlmann, C. Affolter, 2000.
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1. R. M. Silverstein, F. X. Webster, D. J. Kiemle: "Spectrometric Identification of Organic Compounds", Seventh Edition, John Wiley & Sons, Inc., New York, USA, 2005.,
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