COURSE OBJECTIVE: To familiarize students with the fundamentals of microbiology, which include classification of microorganisms, cell structure and composition, physiology, and growth. They will also explain the importance of microorganisms in different ecosystems and the application of microorganisms in environmental protection and industry.
COURSE IMPLEMENTATION PROGRAM
Lectures:
1: Historical overview of microbiology
2: Molecules of living systems
3: Structure of microscope, study of structure of microorganisms
4: Structure and function of prokaryotic cells
5: Structure and function of eukaryotic cells
6: Partial exam
7: Structure and classification of enzymes, mechanism of enzymatic reaction, influence of environmental factors on enzyme activity
8: Energy metabolism
9: Central pathways of energy production
10: Biochemical pathways and mechanisms of selected fermentations and anabolism
11: Determination of metabolic activities and metabolic differences among microorganisms
12: Growth and reproduction of microorganisms
13: Isolation and identification of major groups of indigenous and non-indigenous microorganisms from water, soil and air
14: Suppression of growth of microorganisms
15. Partial exam
Laboratory exercises (block classes of 3 hours each):
1: Microbiology laboratory apparatus and equipment, sterile working techniques
2: Microscope components and working techniques, preparation of microbial specimens
3: Identification of main groups of microorganisms
4: Composition and preparation of nutrient media, quantitative determination of number of microorganisms
5: Inoculation of microorganisms and isolation of pure cultures and their maintenance
6: Staining of microorganisms and individual parts of microbial cells
7: Micrometry and cell size measurement
8: Growth curve of selected microorganisms
9: Study of biochemical activities of microorganisms
10: Ecotoxicity study of organic compounds.
DEVELOPMENT OF GENERAL AND SPECIFIC COMPETENCIES OF STUDENTS:
The student will acquire the ability to recognize the major groups of microorganisms, understand their physiological needs and biochemical capabilities. This basic knowledge will help to master parts of the course in higher years of study where biological processes are interpreted ( Environmental protection, Water, soil and air management, Bioremediation, Industrial wastewater treatment, Solid and hazardous waste treatment, Waste composting).
STUDENT OBLIGATIONS TEACHING AND THEIR PERFORMANCE:
Regular attendance in the lectures will be confirmed by their signature. During the semester they will write two partial exams. Laboratory exercises are compulsory and cannot be reimbursed. They enter the results of each exercise in set templates and must take the final colloquium at the end of the exercises (recognition of specific groups of microorganisms and knowledge of microbiological techniques).
CONDITIONS FOR TO OBTAIN A SIGNATURE:
Completed all laboratory exercises and positively resolved the final pass. Regular attendance at lectures.
TEACHING METHODS:
Lectures and laboratory exercises
METHOD OF EXAM OF KNOWLEDGE AND EXAM:
Two partial exams (following a specified set of materials), recognized as a passing grade if both are positively graded, and a written and oral examination within the prescribed examination time limits.
METHOD OF MONITORING THE QUALITY AND PERFORMANCE OF COURSES:
Student survey
METHODOLOGY PREREQUISITES:
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LEARNING OUTCOMES
Course Learning Outcomes (4-8):
1. identify the microorganisms present in the selected ecosystem
2. distinguish the conditions for optimal growth of microorganisms
3. isolate microorganisms in pure culture for the degradation of pollutants in the environment or the synthesis of specific products
5. investigate models for the growth of microorganisms and evaluate their effectiveness
Program-level learning outcomes:
1. explain the scientific principles important for eco-engineering, especially basic knowledge of chemistry, mathematics, physics, biology and eco-engineering
2. apply basic laboratory skills and work rules in physical, chemical and microbiological laboratories
3. develop awareness and ability to carry out preventive environmental protection measures
4. define simple problems in the field of eco-engineering in order to solve them
Literature:
1. L.M. Prescott, J.P. Harley, D.A. Klein: Microbiology, 3rd edition, Wm.C. Brown Publishers, Boston, 1996.
2. J.N. Lester, J.W. Birkett, Microbiology and Chemistry for Environmental Scientists and Engineers, E& FN SPON, London, 1999.
3. J. Nicklin, K. Graeme-Cook, R. Killington: Microbiology, 2nd edition, BIOS Scientific Publishers Limited , Oxford, 2002.
4. S. Duraković, General Microbiology, Food Technology Engineering, Zagreb, 1996.
5. M. Vuković Domanovac, Teaching materials for the course Microbiology, e-course on the Merlin platform.
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1. L.M. Prescott,, J.P. Harley, D.A. Klein: Microbiology, 3rd Edition, 1996, Wm.C. Brown Publishers, Boston.
2. J.N. Lester, J.W. Birkett, Microbiology and Chemistry for Environmental Scientists and Engineers, E&FN SPON, London, 1999.
3. J. Nicklin, K. Graeme-Cook, R. Killington: Microbiology, 2nd Edition, 2002, BIOS Scientific Publishers Limited, Oxford.,
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Opća mikrobiologija, S. Duraković, Prehrambeno tehnološki inženjering, Zagreb, 1996.
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