1. komponenta

Lecture type	Total
Lectures	20

* Load is given in academic hour (1 academic hour = 45 minutes)

Syllabus:
Chemical sensors and biosensors: definitions, theoretical background, components of a sensing system. Transducers: electrochemical, optical, thermal and mass transducers. Receptor part: mechanism of chemical and biological recognition, biomimetic systems, (bio)chemical selectivity, immobilization techniques, role of functional materials in sensing systems - polymers. Sensor analytical performance: selectivity, sensitivity, precision, accuracy, repeatability, reversibility, response time. Electrochemical sensors and biosensors: potentiometric and amperometric: ionselective electrodes (ISEs), modified electrodes, microelectrodes, standard electrode fabrication techniques; conductometric sensors; FET (field effect transistor) sensors. Optical sensors and biosensors: techniques of optical detection, absorptiometry, UV and visible absorption spectroscopy; reflectometry, luminescence spectroscopy, light scattering techniques, direct and indirect methods, indicator based systems, fiberoptic chemical sensors and biosensors. Mass and thermal sensors.
Learning outcomes:
1. To select the appropriate enzyme, the method for its immobilization as well as the transmitter of electrons from the enzyme to the measuring electrode with respect to the targeted biosensor analysis.
2. To determine the operating mode of the biosensor considering the type of the analyte and interfering substances present in the measured sample.
3. To construct the biosensor for one or severyl analytes.
4. To select the type of transducer (amperometric, potentiometric, optical, temperature, etc.) with respect to the required final characteristics of the sensor.
5. To choose homemade or commercially available sensors and biosensors for use in flowthrough and non flowthrough analytical systems.
6. To design homemade flowthrough and non flowthrough analytical systems based on sensors and biosensors.
Teaching methods:
Lectures, virtual and lab demonstrations, supervisions and discussions, seminar presentations
Examination methods:
Each student will be assigned one real chemical sensor problem usually related to the topic of their PhD thesis. Students have to propose a solution to the problem using knowledge and skills gained during the course. The results have to be presented to the group (20 min. presentation) and in a form of a written report.
Monitoring of the course quality and successfulness: Student Survey

1. Chemical Sensors and Biosensors, B. R. Eggins,, John Wiley & Sons Ltd., New York,, 2002.

D_Izborni - Regular studij - Chemical Engineering and Applied Chemistry