1. komponenta

Lecture type	Total
Lectures	30
Laboratory exercises	15
Seminar	15

* Load is given in academic hour (1 academic hour = 45 minutes)

Course objectives
To introduce students with theory and relevant knowledge of the principles of enzymology that includes the basic properties of enzymes, catalytic mechanisms of enzymes and enzyme kinetics. Students will also be introduced with the techniques of preparation and industrial production of enzymes.

Course content (syllabus)
WEEK 1. Enzyme Properties, nomenclature of enzymes, characteristics and mechanisms.
WEEK 2. Enzyme classes I
WEEK 3. Enzyme classes II
WEEK 4. Enzyme kinetics
WEEK 5. Characterization of enzymes.
WEEK 6. Methods of enzyme production
WEEK 7. Methods of enzyme purification
WEEK 8. Partial exam
WEEK 9. Industrial production of enzymes.
WEEK 10. Kinetic resolution and enantioselectivity of enzymes.
WEEK 11. Reaction media in biocatalysis.
WEEK 12. Heterogeneous biocatalysis.
WEEK 13. Industrial application of enzymes
WEEK 14. Industrial biotransformations.
WEEK 15. Partial exam

Format of instruction:
lectures
seminars and workshops
exercises
independent assignments
laboratory

Student responsibilities
Students are obligated to attend lectures, seminars and laboratory exercises (min 75 %). Students should deliver a lab report, seminar work and give a presentation of their seminar work. Students can pass the exam by taking partial test, or regular written exam.

Monitoring student work
Class attendance
Experimental work
Research
Report
Seminar paper
Preliminary exam
Practical work
Written exam

Learning outcomes at the level of the programme to which the course contributes
- Compile and apply advanced knowledge of natural and technical sciences, particularly chemical engineering and environmental engineering in solving scientific, professional and general social problems.
- Solve engineering problems using the scientific method combining expert knowledge from chemistry, environmental, and chemical engineering as well as material science and engineering.
- Utilise advanced laboratory procedures and instruments for synthesis of new products, create sustainable processes, and solve problems of water, air and soil pollution.
- Apply different analytical techniques, analytical and numerical methods, as well as software tools in creative problem solving of engineering challenges, proposing sustainable technological solutions.
- Independently organise and plan timelines, apply a general methodology for project planning and management in a business environment
- Create a critical analysis, evaluation and interpretation of personal results, and compare them with existing data in scientific and expert literature
- Demonstrate independence and reliability in independent work, as well as effectiveness, reliability and adaptability in team work
- Outline results of independent and teamwork in a written and oral form to non-experts and experts in a clear and coherent way.
- Develop work ethic, personal responsibility and tendency for further skill and knowledge acquisition, according to standards of engineering practice

Expected learning outcomes at the level of the course (3 to 10 learning outcomes)
- Apply knowledge from biotechnology and chemistry to design an environmentally sustainable enzyme process for industrial production of chemicals.
- To differentiate the reaction mechanisms of enzymatically catalyzed reactions for different basic enzyme classes.
- To evaluate the kinetic parameters of the enzyme reaction.
- To identify the advantages and disadvantages of various reaction media for enzyme reactions and decide on favorable reaction conditions for specific cases.
- To compare methods of production, purification, characterization and immobilization of enzymes.

1. Apply knowledge from biotechnology and chemistry to design an environmentally sustainable enzyme process for industrial production of chemicals
2. To differentiate the reaction mechanisms of enzymatically catalyzed reactions for different basic enzyme classes
3. To evaluate the kinetic parameters of the enzyme reaction.
4. To identify the advantages and disadvantages of various reaction media for enzyme reactions and decide on favorable reaction conditions for specific cases
5. To compare methods of production, purification, characterization and immobilization of enzymes

1. Nastavni materijal za predavanja, seminare i vježbe postavljen na mrežnu stranicu kolegija., www.fkit.unizg.hr,
2. Electrochemical methods, Fundamentals and applications, A.J. Bard and L.R. Faulkner, John Wiley & Sons, Inc., New Jersey, 2001.
3. Modern batteries, C.A. Vincent, B. Scrosati, John Wiley & Sons, Inc., New Jersey, 1997.
4. Journal of Power Sources, B. Scrosati, J. Garche, 195 (2010) 2419-2430, 2010.
5. Fuel Cells From Fundamentals to Applications, S. Srinivasan, Springer, New York, 2003.
6. Fuel Cell Engines, M. M. Mench, John Wiley & Sons, Inc., New Jersey, 2008.
7. Electrochemical supercapacitors, Scientific fundamentals and technological applications, B.E. Conway, Springer, 2002.

Izborni predmeti - Regular studij - Chemical and Environmental Technology