1. komponenta

Lecture type	Total
Lectures	20

* Load is given in academic hour (1 academic hour = 45 minutes)

Sample preparation. Importance and basic principle of sample preparation procedure in chromatographic analysis. Goal of sample preparation procedure. Review of recent sample preparation techniques. Extraction as the oldest sample preparation technique.
Analytes in solid samples. Accelerated solvent extraction (ASE). Supercritical fluid extraction (SFE).
Superheated water extraction (SHWE, SWE). Ultrasonic extraction (USE). Microwave assisted solvent extraction (MASE, MAE). Matrix solid-phase dispersion (MSPD). Dispersive solid-phase extraction (DSPE).
Analytes in liquid samples. Solid-phase extraction (SPE). Solid-phase microextraction (SPME). Stir bar sorptive extraction (SBSE). Membrane extraction. Single drop microextraction (SDME). Liquid phase microextraction (LPME).
Analytes in the gas phase.
Another sample preparation techniques. Environmental samples: problems and approach. Specific applications. Optimization of sample preparation procedure. Validation.

1. To connect the fundamental principles of sample preparation with the topic of the candidate's own research with a view to their implementation in the chromatographic analytical procedures.
2. To assess the value of the data obtained by the literature survey in the field of chromatography.
3. To optimize the sample preparation process in order to obtain more meaningful information about the sample.
4. To improve existing or devise new chromatographic analytical methods based on knowledge of modern methods of sample preparation.
5. To compare the advantages and disadvantages of different methods of preparing samples for chromatographic analysis with regard to the physical state of the sample.
6. To analyze problems in chromatographic analysis of samples extracted from the environment based on the state of the sample, the composition of the matrix, and the measured analyte, as well as possible solutions to these problems.

1. Analitika okoliša, skupina autora (ur. M. Kaštelan-Macan, M. Petrović), HINUS i FKIT, Zagreb, 2013.
2. Sample Preparation Techniques in Analytical Chemistry, S. Mitra, Wiley, New Jersey, 2003.
3. Chapter - Sample Preparation for Chromatographic Analysis in: Chromatography Today, C. F. Poole, Elsevier, Amsterdam, 1991.

D_Izborni - Regular studij - Chemical Engineering and Applied Chemistry